Introduction to Upstream Processing Operations

Course Descriptor

This two day course provides an introduction to the typical upstream operations carried out in a biopharamaceutical manufacturing facility. This course will include both hands-on practical and theory components.

During this course trainees will be introduced to cell culturing techniques using small scale shake flasks.  Trainees will then gain hands on practical experience using both stainless steel and disposable bioreactor systems. Trainees will prepare and run a steam in place cycle on a stainless steel bioreactor and then inoculate a production batch into a stainless steel bioreactor.

 

Introduction to Upstream Processing Operations

SKU: upstream-processing-operations Category:

1,300.00

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Dates:
  • 15/04/2019 - 16/04/2019

    8 Places Remaining

Fee:
€1300
Suitable for:

This course is designed for operators, technical staff, engineers and managers wishing to increase their knowledge of the upstream operations in a modern bioprocessing plant.

Who should attend

This course is designed for operators, technical staff, engineers and managers wishing to increase their knowledge of the upstream operations in a modern bioprocessing plant.

Key topics covered

  • Overview of the biopharmaceutical production process
  • Mammalian cell culture aseptic technique
  • Analysis of cell growth and nutrients in culture
  • Cell culture scale up equipment including shake flasks and disposable bioreactors
  • Preparation of a 150L stainless steel bioreactor for steam in place (SIP)
  • Set up and operation of a 30L stainless steel seed bioreactor including inoculation and sampling.

Learning outcomes

Upon successful completion of this module the learner will be able to:

  • Describe the practice of culturing mammalian cells in an aseptic environment from small to large scale
  • List the equipment required for scale up of mammalian cultures
  • Learn  the process of inoculating a stainless steel seed bioreactor.
  • Use GDP to follow and complete SOPs, batch records and log books.
  • Count cells using both an automated and manual method
  • Interpret the nutrient and metabolite levels of cell culture medium
  • Prepare and run an SIP procedure on a 150L stainless steel bioreactor

Assessment

A competency based assessment will be undertaken at the end of the course. This will certify proficiency in the theory and practical skills.

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